Development and validation of detection and control protocols for pathogenic fungi.

Morphological and serological diagnostic protocols for pathogenic fungi are slightly sensitive and they do not allow the detection of the species concerned nor the quantification of the target pathogen. Recently some PCR-based detection techniques have been developed to detect and identify a number of plant pathogenic fungi. These techniques are highly sensitive and reliable, but they are not very effective for quantitative analyses. Most of the disadvantages related  to traditional PCR have been overcome by some adjustments which make it possible to detect the microorganisms through a specific fluorescent signal (real-time PCR).

As a result, the research conducted at MAIB has been directed towards i) delivering real-time PCR-based molecular diagnostic protocols to be used for the quantitative analysis of soil-born and post-harvest pathogens; ii) developing more rapid, simple and reliable detection techniques compared with the traditional ones (1 day for molecular detectin versus 2 weeks or a few months for traditional methods); iii) planning the guidelines for integrated management including also preventive measures.


Starting: 1.01.2006
Scheduled ending: 31.12.2015
Real ending: 23.09.2019
Reference person/s: Yaseen Thaer /
Collaborators: D'Onghia Anna Maria / Valentini Franco /
Thesis: Phenotypic pathogenicity characters of Fusarium spp. on citrus and correlation with phylogenetic effect
Table grapes and Ochratoxin A contamination: an emerging issue
Biological control of soil-borne diseases of olive in the nursery
Biological approaches to the control of olive Verticillium wilt in the nursery
Identification of critical points for Phaeomoniella chlamydospora infection of grapevine propagation materials in nursery
Epidemiology and control of green, blue and whisker mould of citrus and development of Penicillium rot of citrus and development of a molecular detection method for pathogen diagnosis
Observations on the variability of Botryotinia fuckeliana
Evaluation of time-course degradation of R. necatrix DNA in soil by real-time Scorpion PCR
Biological control of phytophthora nicotianae and organic media standardization in citrus nurseries
Ecological and biological investigations on Aureobasidium pullulans, Penicillium spp. and their interaction on citrus fruits
Le brunissement du bois de la vigne : mise au point des methods de diagnostic moléculaires pour Phaeomoniella chlamydospora
Variation in Phomopsis viticola and development of molecular diagnostic tools
Comparison between real-time Scorpion PCR and traditional methods for the detection and quantification of Verticillium dahliae in soils and in infected olive trees
L'ochratoxine A dans le moût et développement des outils moléculaires pour le diagnostic d'Aspergillus carbonarius
Diagnostic en temps réel de P. nicotianae et P. citrophthora à travers la Scorpion PCR
Development and application of a PCR-based method for a rapid detection of Phytophthora nicotianae and P. citrophthora from naturally infected citrus soils
Wine contamination by Ochratoxin A
Genetic variability of Uncinula necator (Schw.) Burr. (Oidium tuckery Berk.)
Institutions involved: Centro di Ricerca e Sperimentazione in Agricoltura “Basile Caramia”, Locorotondo
Centro di ricerca per l’agrumicoltura e le colture mediterranee - Acireale (CT) (Italia)
Università degli Studi della Tuscia
Università degli Studi di Bari
Università degli Studi di Catania
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